| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Megasphaera massiliensis | AATCCGCTTGGACCGCAT | TTGGGAGCGCACGATATGG | 59.73 | 60.23 | 199 | 149 |
100.00%
|
100.00%
|
| Primer 2 | Megasphaera massiliensis | CGCGTCAAGTCGGAAAACC | ACGATTCGCTGCTGCTGT | 59.80 | 60.05 | 177 | 148 |
100.00%
|
100.00%
|
| Primer 3 | Megasphaera massiliensis | ATTGCGCATTCCCGTTGC | CCTGGGCATCGATTTTGGC | 59.82 | 59.56 | 164 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Megasphaera massiliensis | TCCGCAGAATGTCACCGAC | ACGATTCGCTGCTGCTGT | 60.08 | 60.05 | 224 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Megasphaera massiliensis | ATTGCGCATTCCCGTTGC | GCGCACGCAGGGAAATAAG | 59.82 | 59.94 | 185 | 140 |
100.00%
|
100.00%
|
| Primer 6 | Megasphaera massiliensis | GCAGCAATGCTCGACATCC | AGCCGAGTTTACGAGCCTG | 59.65 | 59.78 | 262 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Megasphaera massiliensis | GGTCCAAGCTGCAAAAGGC | TGCTCAACATGCTGCCCA | 60.01 | 59.88 | 251 | 135 |
100.00%
|
100.00%
|
| Primer 8 | Megasphaera massiliensis | CGGTCCAAGCTGCAAAAGG | TGCTCAACATGCTGCCCA | 59.71 | 59.88 | 252 | 135 |
100.00%
|
100.00%
|
| Primer 9 | Megasphaera massiliensis | CCGGTCCAAGCTGCAAAAG | TGCTCAACATGCTGCCCA | 59.71 | 59.88 | 253 | 135 |
100.00%
|
100.00%
|
| Primer 10 | Megasphaera massiliensis | AATCCGCTTGGACCGCAT | ACTGCCGTGATACAGACCG | 59.73 | 59.49 | 267 | 135 |
100.00%
|
100.00%
|